Plant Tissue Culture Techno Commercial Training

Overview
Curriculum
Instructor
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Overview

Step into the cutting edge of botanical science with the Plant Cell & Tissue Culture online course from aprcindia.org. This comprehensive program unveils the principles, techniques, and applications of plant cell and tissue culture, equipping learners to leverage plant biotechnology across agriculture, pharmaceuticals, conservation, and beyond. Learn anytime, anywhere, at your own pace—and turn curiosity into real-world impact.

What you’ll gain

Cutting-Edge Knowledge: Stay ahead with the latest advancements in plant cell and tissue culture.
Diverse Applications: Explore how culture techniques are used in agriculture, pharma, biotech, and conservation.
Career Advancement: Build specialized expertise that enhances opportunities in academia, industry, and agriculture.
Convenience: Flexible online format that fits busy schedules.

Who this course is for

Students and professionals in biology, botany, agriculture, biotechnology, environmental science, and related fields.
Anyone with a passion for plant biology and a desire to explore plant cell and tissue culture.

Prerequisites

Basic understanding of biology and plant science.
Access to a computer with internet connectivity.
Passion for learning and exploring the intricacies of plant biology.

Career scope and opportunities

Research Scientist in biotech companies or research institutions.
Agricultural Biotechnologist working on crop improvement and genetic engineering.
Plant Biotechnologist developing novel plant-based pharmaceuticals.
Conservation Biologist preserving endangered plant species through tissue culture techniques.

Techno-commercial angle: what you’ll learn

Core lab techniques: sterile culture, media preparation, propagation, and maintenance of cultures.
Scale-up and commercialization: moving from lab to market, optimizing processes, and understanding regulatory considerations.
Quality control and biosafety: standards, documentation, and best practices for reliable, compliant work.
Applications for real-world impact: crop improvement, pharmaceutical plant-derived products, and conservation strategies.

Why aprcindia.org

A trusted doorway to practical plant biotechnology education with a focus on both science and commercial applications.
Self-paced online access designed for busy students and professionals.
A curriculum that blends theory with hands-on insights to prepare you for real-world challenges.

Getting started

Ready to explore plant cell and tissue culture with a techno-commercial edge? Enroll in the Plant Cell & Tissue Culture online course at aprcindia.org and transform curiosity into capability.

Learning Outcomes ( Students at the successful completion of the course will be able to )

Comprehend the basic knowledge and applications of plant tissue culture

Identify various facilities required to set up a plant tissue culture laboratory

Acquire a critical knowledge on sterilization techniques related to plant tissue culture

Demonstrate skills of callus culture through hands on experience

Understand the biotransformation technique for production of secondary metabolites
Module1 ( Basic concepts of plant tissue culture )

Plant tissue culture: Definition, history, scope and significance

3 question

30 min

Totipotency, differentiation, dedifferentiation, and re-differentiation; types of cultures - 1) Meristem culture 2) Callus culture 3) Anther culture 4) Embryo culture 5) Ovary culture 6) Ovule culture 7) Pollen culture

Infrastructure and equipment required to establish a tissue culture laboratory - 1) Selection of suitable material 2) Stock plant selection 3) Parts of plant 4) Size of explants 5) Avoid diseased tissue
Module2 ( Sterilization techniques and culture media )

Sterilization techniques and culture media -

3 question

30 min

1) Aseptic conditions – Fumigation, wet and dry sterilization, UV sterilization, ultra filtration 2) Nutrient media: Composition of commonly used nutrient culture media with respect to their contents like inorganic chemicals, organic constituents, vitamins, amino acidsetc 3) Composition and preparation of Murashige and Skoog culture medium
Module3 ( Callus culture technique )

Callus culture technique -

3 question

30 min

1) Explant: Definition, different explants for tissue culture: shoot tip, auxiliary buds, leafdiscs, cotyledons, inflorescence and floral organs, their isolation and surface sterilization; inoculation methods 2) Callus culture: Definition, various steps in callus culture 3) Initiation and maintenance of callus - Growth measurements and subculture; somaclonal variations
Module4 ( Micro propagation )

Micro propagation -

3 question

30 min

1) Direct and indirect morphogenesis, organogenesis, role of PGRs; somaticembryogenesis and synthetic seeds 2) Greenhouse hardening unit operation and management; acclimatization and hardeningof plantlets - need, process, packaging, exports 3) Pathogen (Virus) indexing- significance, methods, advantages, applications
Module5 ( Applications of plant tissue culture )

Applications of plant tissue culture -

3 question

30 min

1) Germplasm conservation: cryopreservation methods, slow growth, applications andlimitations; crayoprotectants 2) Plant transformation techniques and bioreactors; production of secondary metabolites-optimization of yield, commercial aspects, applications, limitations 3) Transgenic plants- gene transfer methods; BT cotton
Module6 ( Benefits of plant tissue culture )

Benefits of plant tissue culture -

3 question

30 min

1) Rapid multiplication of clones 2) Genetic uniformity 3) Aseptic condition 4) Controlled environment
Practical ( Laboratory )

Learning Outcomes: On successful completion of this practical course, student will be able to -

3 question

30 min

1) List out, identify and handle various equipment in plant tissue culture lab 2) Learn the procedures of preparation of media 3) Demonstrate skills on inoculation, establishing callus culture and Micro propagation 4) Acquire skills in observing and measuring callus growth 5) Perform some techniques related to plant transformation for secondary Metabolite production
Plant Tissue Culture ( Practical )

Plant Tissue Culture -

3 question

30 min

1) Principles and applications of- Autoclave, Laminar Airflow, Hot Air Oven 2) Sterilization techniques for glass ware, tools etc 3) MS medium - Preparation of different stock solutions; media preparation 4) Explant preparation, inoculation and initiation of callus from carrot 5) Callus formation, growth measurements 6) Induction of somatic embryos, preparation of synthetic seeds 7) Multiplication of callus and organogenesis 8) Hardening and acclimatization in green house

About the instructor

APRC Expert

Plant Tissue Culture Techno Commercial Training

5 (30+ rating)
67 lectures
10 hr 07 min

APRC (Agricultural Products Research Center) experts are professionals who specialize in helping farmers optimize their production processes, adopt new technology, and implement sustainable practices. With their expertise in agronomy, soil science, crop protection, and farm management, APRC experts play a key role in driving the success and profitability of modern agricultural operations.

APRC experts provide valuable guidance and support to you as they strive to meet industry standards and consumer expectations. So, if you are looking to stay ahead in the rapidly evolving world of agriculture, consider tapping into the knowledge and experience of an APRC expert to ensure your success.

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Overview

What you’ll gain

Who this course is for

Prerequisites

Career scope and opportunities

Techno-commercial angle: what you’ll learn

Why aprcindia.org

Getting started

About the instructor

APRC Expert

Customer Reviews