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Plant Tissue Culture Techno Commercial Training

APRC Course Details
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  • Overview
  • Syllabus
  • Instructor

Why Select Our Plant Tissue Culture Techno Commercial Training?

Step into the cutting edge of botanical science with the Plant Cell & Tissue Culture online course from aprcindia.org. This comprehensive program unveils the principles, techniques, and applications of plant cell and tissue culture, equipping learners to leverage plant biotechnology across agriculture, pharmaceuticals, conservation, and beyond. Learn anytime, anywhere, at your own pace—and turn curiosity into real-world impact.

What you’ll gain

  • Cutting-Edge Knowledge: Stay ahead with the latest advancements in plant cell and tissue culture.
  • Diverse Applications: Explore how culture techniques are used in agriculture, pharma, biotech, and conservation.
  • Career Advancement: Build specialized expertise that enhances opportunities in academia, industry, and agriculture.
  • Convenience: Flexible online format that fits busy schedules.

Who this course is for

  • Students and professionals in biology, botany, agriculture, biotechnology, environmental science, and related fields.
  • Anyone with a passion for plant biology and a desire to explore plant cell and tissue culture.

Prerequisites

  • Basic understanding of biology and plant science.
  • Access to a computer with internet connectivity.
  • Passion for learning and exploring the intricacies of plant biology.

Career scope and opportunities

  • Research Scientist in biotech companies or research institutions.
  • Agricultural Biotechnologist working on crop improvement and genetic engineering.
  • Plant Biotechnologist developing novel plant-based pharmaceuticals.
  • Conservation Biologist preserving endangered plant species through tissue culture techniques.

Techno-commercial angle: what you’ll learn

  • Core lab techniques: sterile culture, media preparation, propagation, and maintenance of cultures.
  • Scale-up and commercialization: moving from lab to market, optimizing processes, and understanding regulatory considerations.
  • Quality control and biosafety: standards, documentation, and best practices for reliable, compliant work.
  • Applications for real-world impact: crop improvement, pharmaceutical plant-derived products, and conservation strategies.

Why aprcindia.org

  • A trusted doorway to practical plant biotechnology education with a focus on both science and commercial applications.
  • Self-paced online access designed for busy students and professionals.
  • A curriculum that blends theory with hands-on insights to prepare you for real-world challenges.

Hands-on Practical Plant Tissue Culture Training (2 Days)

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Lab /Practical Fees will be extra on course fees

Hands-on Practical Plant Tissue Culture Training
  • After take online class
  • 2 Days

Learning Outcomes: On successful completion of this practical course, student will be able to:

  • List out, identify and handle various equipment in plant tissue culture lab
  • Learn the procedures of preparation of media
  • Demonstrate skills on inoculation, establishing callus culture and Micro propagation
  • Acquire skills in observing and measuring callus growth
  • Perform some techniques related to plant transformation for secondary Metabolite production
  • Principles and applications of- Autoclave, Laminar Airflow, Hot Air Oven
  • Sterilization techniques for glass ware, tools
  • Medium - Preparation of different stock solutions; media preparation
  • Explant preparation, inoculation and initiation of callus from carrot
  • Callus formation, growth measurements
  • Induction of somatic embryos, preparation of synthetic seeds
  • Multiplication of callus and organogenesis
  • Hardening and acclimatization in green house
  • Module I
    Structure of the Module
    2 Days
    1 hour/day
    Comprehend the basic knowledge and applications of plant tissue culture
    Identify various facilities required to set up a plant tissue culture laboratory
    Acquire a critical knowledge on sterilization techniques related to plant tissue culture
    Demonstrate skills of callus culture through hands on experience
    Understand the biotransformation technique for production of secondary metabolites
  • Module II
    Structure of the Module
    2 Days
    1 hour/day
    Plant tissue culture: Definition, history, scope and significance
    Totipotency, differentiation, dedifferentiation, and re-differentiation; types of cultures - 1) Meristem culture 2) Callus culture 3) Anther culture 4) Embryo culture 5) Ovary culture 6) Ovule culture 7) Pollen culture
    Infrastructure and equipment required to establish a tissue culture laboratory - 1) Selection of suitable material 2) Stock plant selection 3) Parts of plant 4) Size of explants 5) Avoid diseased tissue
  • Module III
    Structure of the Module
    2 Days
    1 hour/day
    Sterilization techniques and culture media -
    1) Aseptic conditions – Fumigation, wet and dry sterilization, UV sterilization, ultra filtration 2) Nutrient media: Composition of commonly used nutrient culture media with respect to their contents like inorganic chemicals, organic constituents, vitamins, amino acidsetc 3) Composition and preparation of Murashige and Skoog culture medium
  • Module IV
    Structure of the Module
    2 Days
    1 hour/day
    Callus culture technique -
    1) Explant: Definition, different explants for tissue culture: shoot tip, auxiliary buds, leafdiscs, cotyledons, inflorescence and floral organs, their isolation and surface sterilization; inoculation methods 2) Callus culture: Definition, various steps in callus culture 3) Initiation and maintenance of callus - Growth measurements and subculture; somaclonal variations
  • Module V
    Structure of the Module
    2 Days
    1 hour/day
    Micro propagation -
    1) Direct and indirect morphogenesis, organogenesis, role of PGRs; somaticembryogenesis and synthetic seeds 2) Greenhouse hardening unit operation and management; acclimatization and hardeningof plantlets - need, process, packaging, exports 3) Pathogen (Virus) indexing- significance, methods, advantages, applications
  • Module VI
    Structure of the Module
    2 Days
    1 hour/day
    Applications of plant tissue culture -
    1) Germplasm conservation: cryopreservation methods, slow growth, applications andlimitations; crayoprotectants 2) Plant transformation techniques and bioreactors; production of secondary metabolites-optimization of yield, commercial aspects, applications, limitations 3) Transgenic plants- gene transfer methods; BT cotton
  • Module VII
    Structure of the Module
    2 Days
    1 hour/day
    Benefits of plant tissue culture -
    1) Rapid multiplication of clones 2) Genetic uniformity 3) Aseptic condition 4) Controlled environment

About the instructor

instructor

APRC Expert

Plant Tissue Culture Techno Commercial Training
  • 5 (30+ rating)
  • 67 lectures
  • 10 hr 07 min

APRC (Agricultural Products Research Center) experts are professionals who specialize in helping farmers optimize their production processes, adopt new technology, and implement sustainable practices. With their expertise in agronomy, soil science, crop protection, and farm management, APRC experts play a key role in driving the success and profitability of modern agricultural operations.

APRC experts provide valuable guidance and support to you as they strive to meet industry standards and consumer expectations. So, if you are looking to stay ahead in the rapidly evolving world of agriculture, consider tapping into the knowledge and experience of an APRC expert to ensure your success.

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₹25000.00
  • Level
    Startup Course
  • Lectures
    30 Lectures
  • Duration
    20h 30m
  • Category
    Plant Tissue Culture
  • Language
    English Hindi Bengali
  • Certificate
    Yes
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